Fig. 4

Candidate SNPs in the HELB gene. a Representation of the HELB gene based on human hg19 coordinates (chr12: 66,696,335–66,737,423) and bovine bosTau6 coordinates (chr5:47,713,179–47,751,456). b Multiple sequence alignment of amino acids across representative mammalian species. In red: the candidate indicine-specific mutations. c HELB amino acid sequence with the indicine-specific mutations are highlighted in red. Non-synonymous mutations with substitution residues are located at positions 10, 788 and 791. In purple, residue 428, which had been previously associated to temperature sensitive murine cell lines [88]. d Allele frequency of the SNP rs447470311 located in chr5:47726121 across the 36 breeds in Run6 1000 Bull Genomes Project which contain the G variant [24]